Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide -phosphate derivative
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چکیده
منابع مشابه
Nucleotide-mimetic synthetic ligands for DNA-recognizing enzymes One-step purification of Pfu DNA polymerase.
The commercial availability of DNA polymerases has revolutionized molecular biotechnology and certain sectors of the bio-industry. Therefore, the development of affinity adsorbents for purification of DNA polymerases is of academic interest and practical importance. In the present study we describe the design, synthesis and evaluation of a combinatorial library of novel affinity ligands for the...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkq1053